Application and Advantages of ELISPOT Differences between ELISPOT assays and other approaches for measuring antigen- specific T cell immunity
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چکیده
Subsequently, the frequencies of T cells recognizing individual antigens are very low. As a result of the clonal expansion associated with infections, the frequency of antigen-specific effector cells can transiently rise to as high as 1:100, but these frequencies typically settle in the range of 1:10,000 for effector memory or memory cells. The frequencies of specific T cells in blood after immunizations with subunit vaccines (proteins/peptides), environmental sensitization and in long term anti-microbial immunity rarely exceed the 1:10,000 thresholds.
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